Glutathione Peroxidase from bovine erythrocytes
SIGMA/G6137 - lyophilized powder, ≥300 units/mg protein
Synonym: GSH-Px; Glutathione:hydrogen-
CAS Number: 9013-66-5
EC Number: 232-749-6
MDL Number: MFCD00131195
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| biological source | bovine erythrocytes |
| composition | Protein, 10-30% modified Warburg-Christian |
| form | lyophilized powder |
| mol wt | 84.5 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥300 units/mg protein |
| storage temp. | −20°C |
| Application: | Glutathione Peroxidase from bovine erythrocytes has been used as an oxygen radical scavenger to study its effect on cytotoxicity of 1,3-dilinoleoylglycerol (DLG) against E1A-3Y1 cells. |
| Application: | Glutathione peroxidase from bovine erythrocytes was used as a positive control in cloning and characterization of full-length cDNAs encoding two glutathione peroxidases (GpXs) from Globodera rostochiensis. It was used for the determination of glutathione peroxidase activity in human milk. |
| Biochem/physiol Actions: | Glutathione peroxidase helps to reduce (peroxides) H2O2 to water and lipid peroxides to lipid alcohols. |
| Biochem/physiol Actions: | Glutathione peroxidase is an enzyme which reduced lipid hydroperoxides into their corresponding alcohols. It also reduces free hydrogen peroxide in to water. In vivo it is responsible for protecting hemoglobin from oxidative breakdown. |
| Biochem/physiol Actions: | Protects cells against oxidative damage by catalyzing the reduction of hydrogen peroxide in the presence of reducing agent glutathione. In cellular membranes it may induce lipid peroxidation through the reduction of hydrogen peroxide or polyunsaturated fatty acid hydroperoxides. |
| General description: | Glutathione peroxidase is an antioxidant enzyme that contains selenium. It is present in the glandular epithelium of human endometrium. |
| Other Notes: | Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0. |
| Other Notes: | One unit will catalyze the oxidation by H2O2 of 1.0 μmole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 °C. |
| Packaging: | 100, 200, 500 units in serum bottle |
| Physical form: | Lyophilized powder containing 25% sucrose and 2.5% dithiothreitol with sodium phosphate buffer salts |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥300 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.11.1.9 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |