L-Glutamic acid γ-(p-nitroanilide) hydrochloride
SIGMA/G6133 - γ-glutamyl transpeptidase substrate
Synonym: L-γ-Glutamyl-p-nitroanilide
CAS Number: 67953-08-6
Empirical Formula (Hill Notation): C11H13N3O5 · HCl
Molecular Weight: 303.70
EC Number: 267-944-5
MDL Number: MFCD00039054
Linear Formula: C11H13N3O5 · HCl
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G6133-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G6133-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G6133-5G
| assay | ≥98% (HPLC) |
| form | powder |
| InChI | 1S/C11H13N3O5.ClH/c12-9(1 |
| InChI key | OJEVFSFTVARWQX-UHFFFAOYSA |
| Quality Level | 300  |
| SMILES string | Cl.NC(CCC(=O)Nc1ccc(cc1)N |
| solubility | water: 5 mg/mL, clear, yellow |
| storage temp. | 2-8°C |
| Application: | L-Glutamic acid γ-(p-nitroanilide) hydrochloride has been used as a solute carrier family 1 member 5 (SLC1A5) inhibitor: • or an inhibitor of the cell membrane glutamine transporter to study the effects of blocking glutamine uptake on esophageal adenocarcinoma (EACC) and thioredoxin-interacting protein (TXNIP) • in glutamine ELISA assay to treat confluent differentiated uninfected human colonoid monolayer (HCM) in apical and basolateral compartments to study its effects • to study its effect on SLC1A5_var-mediated mitochondrial glutamine transport inhibition • to study its effects on cellular glutathione levels, cellular reactive oxygen species (ROS) levels, and mitochondrial ROS levels |
| Biochem/physiol Actions: | L-Glutamyl-p-nitroanilide (GPNA) is commonly used to block the glutamine (Gln) transporter alanine-serine-cysteine transporter 2 (ASCT2). It can also inhibit sodium-dependent and independent amino acid transporters. GPNA, γ-glutamyltransferase (GGT) substrate plays a key role in the hydrolysis of its γ-glutamyl bond and the subsequent release of the chromogen, p-nitroaniline (PNA). |
| General description: | Substrate for γ-glutamyl transpeptidase |
| Packaging: | 1, 5, 25 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |