PNGase F from Elizabethkingia miricola
SIGMA/G5166 - buffered aqueous solution
Synonym: N-Glycosidase F; Peptide N-glycosidase
CAS Number: 83534-39-8
MDL Number: MFCD06656235
Product Type: Chemical
Figure 4. Cleavage site and structural requirements for PNGase F.
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
R3 = H or α(1→6)fucose
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
R3 = H or α(1→6)fucose
| biological source | bacterial (Elizabethkingia miricola) |
| conjugate | (N-linked) |
| form | buffered aqueous solution |
| mol wt | 36 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| specific activity | ≥20000 units/mg protein |
| storage temp. | 2-8°C |
| Application: | PNGase F from Elizabethkingia miricola has been used for the deglycosylation of the proteins. |
| Application: | Used to deglycosylate protein. |
| Biochem/physiol Actions: | Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. |
| General description: | PNGase F (Peptide N-glycosidase F) cleaves asparagine-linked glycoproteins, which produces carbohydrate-free peptides and detached full-length oligosaccharides. |
| Other Notes: | One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit. |
| Physical form: | Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.5.1.52 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |