α-Glucosidase from Saccharomyces cerevisiae
SIGMA/G5003 - Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)
Synonym: α-D-Glucosidase; α-D-Glucoside glucohydrolase; Maltase from yeast
CAS Number: 9001-42-7
EC Number: 232-604-7
MDL Number: MFCD00081321
Product Type: Chemical
Unit Definition One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.
Stoichiometry: Maltose + H2O → 2 D-Glucose
12 Principles of Green Chemistry: Principle 1—Waste Prevention. This product has a smaller environmental footprint through reduction of waste in the manufacturing process.
| composition | Protein, ≥50% |
| contains | phosphate buffer salts and EDTA as balance |
| foreign activity | β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1% |
| form | lyophilized powder |
| greener alternative category |  , Enabling |
| greener alternative product characteristics | Waste Prevention Design for Energy Efficiency Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| relevant disease(s) | diabetes (management of non-insulin-dependent diabetes mellitus) |
| specific activity | ≥10 units/mg protein (using p-nitrophenyl α- |
| storage temp. | −20°C |
| sustainability | Greener Alternative Product |
| type | Type I |
| UniProt accession no. | P38158  |
| P53341 |
| Analysis Note: | Protein determined by biuret. |
| Application: | For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters |
| Application: | α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition. |
| Biochem/physiol Actions: | Hydrolysis of terminal, non-reducing 1→4-linked |
| Biochem/physiol Actions: | α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles . |
| Other Notes: | One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α- |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| Packaging: | 100 units in glass bottle |
| Packaging: | 1000 units in poly bottle |
| Packaging: | Sold on basis of p-nitrophenyl α- |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.20 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |