Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse
SIGMA/G3893 - clone G-A-5, ascites fluid
Synonym: Anti Gfap; Anti-Gfap; GFAP Antibody - Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse; Gfap Antibody; Glial Fibrillary Acidic Protein Antibody; Anti-GFAP
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: The impact of Bdnf gene deficiency to the memory impairment and brain pathology of APPswe/PS1dE9 mouse model of Alzheimer′s disease. By: Rantamäki, T., Kemppainen, S., et al. in PLoS One, 2013. PubMed ID: 23844236 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0068722), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Primary neurons isolated from the hippocampus of a day 18 embryonic Sprague Daley rat stained with Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP), clone G-A-5 (Cat. No. G3893) at a 1:600 dilution (green), Rabbit Anti-Neurofilament (red) and bisBenzimide (blue). From Kenneth Reese, BrainBits, Springfield, IL.
Immunofluorescence
Microglia in the hippocampus (CA3 region) in rat brain sections are stained with Monoclonal Anti-Glial Fibrillary Acidic Protein, clone No. G-A-5 (Cat. No. G3893) and Goat Anti-Mouse IgG, TRITC (Cat. No. T6528).
From Dr. S. Shoham, Herzog Hospital, Dept of Research, Jerusalem, Israel.
Microglia in the hippocampus (CA3 region) in rat brain sections are stained with Monoclonal Anti-Glial Fibrillary Acidic Protein, clone No. G-A-5 (Cat. No. G3893) and Goat Anti-Mouse IgG, TRITC (Cat. No. T6528).
From Dr. S. Shoham, Herzog Hospital, Dept of Research, Jerusalem, Israel.
Immunohistochemistry
Astrocytes in the pyriform cortex of rat brain sections are stained with Monoclonal Anti-Glial Fibrillary Acidic Protein, clone no. G-A-5 (Cat. No. G3893) and ExtrAvidin® Peroxidase Staining Kit (Cat. No. EXTRA2).
From Dr. S. Shoham, Herzog Hospital, Dept of Research, Jerusalem, Israel.
Astrocytes in the pyriform cortex of rat brain sections are stained with Monoclonal Anti-Glial Fibrillary Acidic Protein, clone no. G-A-5 (Cat. No. G3893) and ExtrAvidin® Peroxidase Staining Kit (Cat. No. EXTRA2).
From Dr. S. Shoham, Herzog Hospital, Dept of Research, Jerusalem, Israel.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat brain sections with 0.05 μg/mL Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, Clone: G-A-5 (Cat. No. G3893). The antibody was developed using goat-Anti-Mouse IgG, DyLight 594 conjugate. Slides were counterstained with DAPI (blue) to stain nuclei. (The picture is courtesy of Fabrice GANKAM MD, Ph.D., Néphrologie Dialyse et Transplantation, Hopital Erasme - ULB)
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of rat cerebellum stained with 1:400 Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) clone No. G-A-5 antibody produced in mouse (Cat. No. G3893) followed by Biotin (Cat. No. B7151) and by ExtrAvidin®-Peroxidase (Cat. No. C2886).
Immunoblotting Whole extract of rat B35 cells was separated on SDS-PAGE and probed with Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP), Clone: G-A-5 (Cat. No. G3893). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:250 2. Antibody dilution 1:500 3. Antibody dilution 1:1,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | G-A-5, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: suitable |
| immunocytochemistry: suitable | |
| immunohistochemistry: 1:400 using rat brain sections (alcohol-fixed) | |
| indirect immunofluorescence: suitable | |
| microarray: suitable | |
| UniProt accession no. | P14136  |
| Application: | Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse is suitable for immunohistochemistry at a working dilution of 1:400 using rat brain sections (alcohol-fixed) and microarray analysis. It may be used for immunocytochemical localization of GFAP in human, pig, and rat tissues. It is also suitable for localization of GFAP using immunoblot assays. In indirect immunofluorescent labeling on alcohol-fixed or frozen sections, this antibody stains astrocytes and Bergmann glia cells, gliomas, and other glial cell derived tumors. The antibody was used as a primary antibody in immunocytochemistry analysis: • of primary cerebral microvascular EC cultures to study the effect of microglia on the BBB (blood-brain barrier) and its primary constituents • to study the wound healing effects of matrix metalloproteinase-2 that promote recovery after spinal cord injury • to study the negative regulation of embryonic neural progenitor cell proliferation by Toll-like receptor 3 |
| Biochem/physiol Actions: | The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, Bergmann glia cells and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections. |
| Biochem/physiol Actions: | The gene GFAP encodes an intermediate filament protein (50kDa) of mature astrocytes, which may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. Defects in this gene causes Alexander disease. It is a rare disorder of astrocytes in the CNS. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal anti-glial fibrillary acidic protein (GFAP) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP is the intermediate filament expressed in astrocytes. The gene encoding it is localized on human chromosome 17q21.31. The isotype is determined using ImmunoTypeTM Kit (Product Code ISO-1) and by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Product Code ISO-2). Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological, and biochemical criteria. Intermediate filaments differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. GFAP (molecular weight of 50 kDa) is the cell specific IF protein in astrocytes. |
| Immunogen: | GFAP from pig spinal cord |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) is provided as ascites fluid containing 15 mM sodium azide as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |