Guanidine hydrochloride
SIGMA/G3272 - Molecular Biology, ≥99%
Synonym: Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride
CAS Number: 50-01-1
Empirical Formula (Hill Notation): CH5N3 · HCl
Molecular Weight: 95.53
EC Number: 200-002-3
MDL Number: MFCD00013026
Linear Formula: NH2C(=NH)NH2 · HCl
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: G3272-100G
This image depicts SKU: G3272-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: G3272-500G
This image depicts SKU: G3272-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: G3272-1KG
This image depicts SKU: G3272-1KG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: G3272-2KG
This image depicts SKU: G3272-2KG
| assay | ≥99% |
| biological source | synthetic (organic) |
| cation traces | Pb: ≤5 ppm |
| color | white |
| density | 1.3 g/cm3 (lit.) |
| foreign activity | DNase, RNase, none detected |
| form | crystalline powder |
| grade | Molecular Biology |
| impurities | ≤0.3% water (Karl Fischer) |
| InChI | 1S/CH5N3.ClH/c2-1(3)4;/h( |
| InChI key | PJJJBBJSCAKJQF-UHFFFAOYSA |
| mp | 180-185 °C (lit.) |
| pH | (25 °C, 4.6 - 6/573 g/L) |
| Quality Level | 400  |
| SMILES string | Cl[H].NC(N)=N |
| solubility | H2O: 6 M, clear, colorless |
| storage condition | ( |
| technique(s) | RNA extraction: suitable |
| UV absorption | λ: 260 nm Amax: ≤0.10 |
| λ: 290 nm Amax: ≤0.05 |
| Application: | Guanidine hydrochloride has been used: • as a component of the extraction buffer for the extraction of proteoglycans • in extraction during protein fractionation of ATDC5 cell lines • as a chemical additive to study its effective absorbance spectra in structural analysis • to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer • as a component of the 2,4-dinitrophenylhydrazin • in RNA isolation to dissociate nucleoproteins and inhibit RNase |
| Application: | Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. |
| Biochem/physiol Actions: | Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain. It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1). |
| Disclaimer: | May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. |
| Features and Benefits: | • Highly versatile surfactant for your cell biology and biochemical research • Suitable for sensitive molecular biology applications • Free from Exonuclease, Dnase and Rnase • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications |
| General description: | Guanidine hydrochloride, also known as aminoformamidine hydrochloride, is a versatile compound widely used in molecular biology and biochemical research. Functioning as a potent chaotropic agent, it disrupts protein and nucleic acid structures, making it invaluable for purifying proteins, particularly mRNA. Its denaturing capabilities extend to unfolding proteins, aiding their purification and subsequent refolding, and it can even solubilize denatured insoluble proteins at higher concentrations. At lower concentrations, guanidine hydrochloride exhibits a unique effect, promoting the refolding of denatured proteins and restoring enzymatic activity. This property is advantageous for protein renaturation studies. In RNA extraction, guanidine hydrochloride acts as a robust denaturant, disrupting cell structures and inactivating RNA enzymes, ensuring the integrity of extracted RNA. In summary, guanidine hydrochloride is a versatile compound with applications in protein purification, nucleic acid isolation, and protein refolding studies, showcasing its multifaceted utility in molecular biology and biochemical research. |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 1, 2 kg in poly bottle |
| Packaging: | 100, 500 g in poly bottle |
| Preparation Note: | In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 + H332 - H315 - H319 |
| Precautionary statements | P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38 |
| Safety Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99% |
| mp | 180-185 °C (lit.) |
| Density | 1.3 g/cm3 (lit.) |
| UNSPSC | 12352107 |