L-Glutamic Dehydrogenase from bovine liver

SIGMA/G2626 - Type II, 50% glycerol solution, ≥35 units/mg protein

Synonym: L-GLDH; L-Glutamate:NAD[P]⁺ Oxidoreductase (deaminating); Glutamate Dehydrogenase from bovine liver

CAS Number: 9029-12-3
MDL Number: MFCD00131461
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G2626-20MG	20 mg	$100.00 1/EA	Add To Favorites
45-G2626-50MG	50 mg	$177.00 1/EA	Add To Favorites
45-G2626-100MG	100 mg	$279.00 1/EA	Add To Favorites
45-G2626-500MG	500 mg	$949.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

biological source	bovine liver
form	glycerol solution (50%)
mol wt	310-350 kDa
Quality Level	200
shipped in	wet ice
specific activity	≥35 units/mg protein
storage temp.	2-8°C
type	Type II
UniProt accession no.	P00366

Analysis Note:	Protein determined by biuret.
Application:	L-glutamic dehydrogenase was used to catalyze the conversion of isocitrate into α-ketoglutarate and carbon dioxide.
Biochem/physiol Actions:	Mammalian forms of this enzyme, including this bovine form, can use either NADP(H) or NAD(H) as coenzymes. L-glutamic dehydrogenase plays a unique role in mammalian metabolism. The reverse reaction catalyzed by this enzyme is the only pathway by which ammonia can become bound to the α-carbon atom of an α-carboxylic acid and thus, is the only source of de novo amino acid synthesis in mammalian species. The bovine enzyme is characterized by three sets of properties: • It has a reversible concentration-dependent association, producing higher molecular weight forms. • Forms tight enzyme-reduced coenzyme-substrate ternary complexes whose rates of dissociation modulate the steady-state reaction rates. • Exhibits a wide variety of effects from the binding of any of a number of nucleotide modifiers. L-glutamic dehydrogenase catalyzes the conversion of glutamate to α-ketoglutarate.
Other Notes:	One unit will reduce 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.3 at 25 °C, in the presence of ammonium ions.
Physical form:	50% glycerol solution

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥35 units/mg protein
Storage Temp.	2-8°C
Enzyme Commission (EC) Number	1.4.1.3 ( BRENDA \| IUBMB )
UNSPSC	12352204

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