Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal
SIGMA/G1546 - clone GSN149, purified from hybridoma cell culture
Synonym: Mouse Anti-GFP
Product Type: Chemical
Western Blotting JOURNAL CITATION: The immunosuppressive agent mizoribine monophosphate is an inhibitor of the human RNA capping enzyme. By: Picard-Jean, F., Bougie, I., et al. in PLoS One, 2013. PubMed ID: 23349942 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0054621), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting
Recombinant GFP protein was separated on SDS-PAGE and blotted with Monoclonal Anti-Green Fluorescent Protein (2 μg/mL) (Cat. No. G1546) The antibody was developed with Anti-Mouse IgG, Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Recombinant GFP protein was separated on SDS-PAGE and blotted with Monoclonal Anti-Green Fluorescent Protein (2 μg/mL) (Cat. No. G1546) The antibody was developed with Anti-Mouse IgG, Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Immunoblotting Cell extracts of HEK- 293T cells transfected with a GFP-PRMT1 mammalian expression vector were separated on SDS-PAGE and blotted with Monoclonal Anti-GFP C-terminal (2 μg/mL down to 0.25 μg/mL). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1: 2 μg/mL antibody 2: 1 μg/mL antibody 3: 0.5 μg/mL antibody 4: 0.25 μg/mL antibody 5: Negative control (no antibody)
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GSN149, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. • Immunocytochemistry • Immunofluorescence-cell culture cells • Immunoprecipitation • Western Blotting • Western blotting following immunoprecipitation |
| Application: | Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse was used in surface plasmon resonance (SPR) gold chip analysis to study the cysteine- and domain-mediated immobilization of enhanced GFP model protein. |
| Biochem/physiol Actions: | GFP is implicated in protein detection and localization in living cells, as well as gene expression monitoring in prokaryotes and eukaryotes. It detects protein-protein interactions in living cells. |
| Biochem/physiol Actions: | Green fluorescent protein, produced in the jellyfish, Aequorea victoria, acts as secondary fluorescent protein in an energy transfer reaction to produce green light. It ability to produce a highly visible, internal fluorophore is widely applicable as marker for gene expression and to produce chimeric proteins to understand protein biochemistry. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the hybridoma GSN149 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of the Green fluorescent protein from jellyfish Aequorea victoria. GFP is a 27 kDa protein (238 amino acids) that can produce a strong green fluorescence without the need for a substrate. It absorbs light maximally at 395 nm and emits a bright green fluorescence with a peak at 509 nm. The GFP chromophore is formed through cyclization and oxidation of an internal tripeptide motif (Ser65, Gly69 and Tyr66). |
| General description: | The antibody reacts specifically with GFP-fusion proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |