Anti-GFP, N-terminal antibody produced in rabbit
SIGMA/G1544 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Green Fluorescent Protein
MDL Number: MFCD05665217
Product Type: Chemical
Western Blotting JOURNAL CITATION: Analysis of the cytoprotective role of α-crystallins in cell survival and implication of the αA-crystallin C-terminal extension domain in preventing Bax-induced apoptosis. By: Hamann, S., Métrailler, S., et al. in PLoS One, 2013. PubMed ID: 23383327 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0055372), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: A dimerized HMX1 inhibits EPHA6/epha4b in mouse and zebrafish retinas. By: Marcelli, F., Boisset, G., et al. in PLoS One, 2014. PubMed ID: 24945320 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0100096), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Analysis of the cytoprotective role of α-crystallins in cell survival and implication of the αA-crystallin C-terminal extension domain in preventing Bax-induced apoptosis. By: Hamann, S., Métrailler, S., et al. in PLoS One, 2013. PubMed ID: 23383327 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0055372), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting
Decreasing amount of recombinant GFP were loaded onto lanes of a mini gel separated on SDS-PAGE, blotted with Rabbit Anti-GFP (N-Terminal) (Cat. No. G1544) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Protein loaded per lane ranged from 3−200 ng.
Decreasing amount of recombinant GFP were loaded onto lanes of a mini gel separated on SDS-PAGE, blotted with Rabbit Anti-GFP (N-Terminal) (Cat. No. G1544) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Protein loaded per lane ranged from 3−200 ng.
Immunoblotting Whole extract of human HEK-293T cells over-expressing N-Terminal GFP tagged fusion protein was separated on SDS-PAGE and probed with Rabbit Anti-GFP, N-Terminal (Cat. No. G1544). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.04 μg/mL antibody 2. Negative Control: without first antibody
Immunoblotting Whole extract of human HEK-293T cells over-expressing C-Terminal GFP tagged fusion protein was separated on SDS-PAGE and probed with Rabbit Anti-GFP, N-Terminal (Cat. No. G1544). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.04 μg/mL antibody 2. Negative Control: without first antibody
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates |
| western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts |
| Application: | Anti-GFP, N-terminal antibody produced in rabbit has been used: • in immunoblotting • in the histochemistry and immunohistochemistry • in the immunoprecipitation |
| Biochem/physiol Actions: | Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins. |
| Biochem/physiol Actions: | Specific for GFP fusion proteins. |
| General description: | Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln |
| Immunogen: | synthetic peptide corresponding amino acids 3-17 of GFP. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |