DMT-dG(dmf) Phosphoramidite

SIGMA/G115060 - configured for ABI

Synonym: DMT-dG(dmf) Amidite; N-[(dimethylamino)methylene]-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxyguanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]; N2-Dimethylformamidine-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine-3′-O-[O-(2-cyanoethyl)-N,N′-diisopropylphosphoramidite]

CAS Number: 330628-04-1
Empirical Formula (Hill Notation): C₄₃H₅₃N₈O₇P
Molecular Weight: 824.90
MDL Number: MFCD00797420; MFCD00797420
Linear Formula: C₄₃H₅₃N₈O₇P
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G115060-12X1G	1 g	$669.00 12/EA	Add To Favorites
45-G115060-12X3G	3 g	$1870.00 12/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

λ	conforms (UV/VIS Identity)
assay	≥99% (³¹P-NMR)
	≥99.0% (reversed phase HPLC)
color	white to off-white
compatibility	configured for ABI
form	powder
InChI	1S/C43H53N8O7P/c1-29(2)51(30(3)4)59(56-24-12-23-44)58-36-25-38(50-28-45-39-40(50)47-42(48-41(39)52)46-27-49(5)6)57-37(36)26-55-43(31-13-10-9-11-14-31,32-15-19-34(53-7)20-16-32)33-17-21-35(54-8)22-18-33/h9-11,13-22,27-30,36-38H,12,24-26H2,1-8H3,(H,47,48,52)/b46-27-/t36-,37+,38+,59?/m0/s1
InChI key	YRQAXTCBMPFGAN-UJASEYITSA-N
nucleoside profile	base: deoxyguanosine base protecting group: DMF 2' protecting group: none 5' protecting group: DMT deprotection: fast/standard
product line	Proligo Reagents
Quality Level	200
SMILES string	COc1ccc(cc1)C(OC[C@H]2O[C@H](C[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)n3cnc4C(=O)NC(N=C/N(C)C)=Nc34)(c5ccccc5)c6ccc(OC)cc6
storage temp.	-10 to -25°C
technique(s)	oligo synthesis: suitable
type	for DNA synthesis

General description:

"Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamidine (dmf) baseprotecting group enables the rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.Key Features of dG(dmf) :

• dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °C
• The dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomer
• dG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites
• dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite
• No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for ABI Synthesizers."

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
Storage Temp.	-10 to -25°C
UNSPSC	12352200

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