Glutathione High Capacity Magnetic Agarose Beads
SIGMA/G0924 - (1:1 suspension in a 30% ethanol solution)
Synonym: Magnetic agarose beads
MDL Number: MFCD00131200
Product Type: Chemical
| analyte chemical class(es) | proteins (GST) |
| capacity | ≥15 μmol/mL, agarose binding capacity |
| conjugate | magnetic beads |
| form | (1:1 suspension in a 30% ethanol solution) |
| matrix | 4% beaded magnetic agarose |
| matrix spacer | 12 atoms |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | immunoprecipitation (IP): suitable |
| protein purification: suitable |
| Application: | Useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as recombinant GST-tagged fusion proteins or native glutathione S-transferase, glutathione peroxidase and glyoxalase I from cell lysates and other biological samples. Can be used for purification in single tubes or for high throughput in 96-well multiwell plates. The magnetic properties of the beads aid in manipulations, such as repetitive washings and recovery of the protein. This leads to greater experimental reproducibility and more accurate quantitation of the proteins of interest. |
| General description: | Glutathione Magnetic Agarose Beads consist of a paramagnetic 4% beaded agarose. Glutathione is covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom (10 carbon) spacer. The Glutathione Magnetic Agarose Beads are useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as native glutathione S-transferase (GST), glutathione peroxidase, and glyoxalase I, or GST-tagged recombinant fusion proteins from cell lysates or other biochemical solutions while exhibiting low non-specific binding of other proteins. Recombinant GST-tagged proteins, bound to the affinity resin are separated with the use of a magnet. The magnetic properties allow for very rapid processing, as well as aid in manipulations, such as repetitive washings and recovery of the desired protein. This leads to faster recovery, experimental reproducibility, and more accurate quantitation of the proteins of interest. The matrix of the magnetic beads is a 4% beaded agarose with an average diameter of 50 μ and a diameter range of 20-75 μ. Paramagnetic iron is impregnated within the beads. |
| Physical form: | 1:1 suspension in a 30% ethanol solution |
| Symbol |  GHS02 |
| Signal word | Danger |
| Hazard statements | H225 |
| Precautionary statements | P210 - P233 - P240 - P241 - P242 - P243 |
| RIDADR | UN1170 - class 3 - PG 3 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |