Granulocyte-Macrophage Colony-Stimulating Factor from rat

SIGMA/G0792 - GM-CSF, recombinant, expressed in E. coli

Synonym: GM-CSF

MDL Number: MFCD01868356
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-G0792-5UG	5 µg	$617.00 1/EA		Add To Favorites
45-G0792-5X5UG	5 µg	$1700.00 1/EA		Add To Favorites

This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

Properties
Descriptions
Safety Info.
Basic Data

assay	>97% (SDS-PAGE)
biological source	rat
form	lyophilized powder
impurities	<1 EU/μg
mol wt	14.7 kDa
packaging	pkg of 5 μg
	pkg of 5X5 μg
potency	0.03-0.1 ng/mL
quality	endotoxin tested
Quality Level	100
recombinant	expressed in E. coli
storage condition	avoid repeated freeze/thaw cycles
storage temp.	−20°C
UniProt accession no.	P48750

Analysis Note:	The biological activity is measured by its ability to induce proliferation of the factor-dependent mouse cell line, DA3.
Biochem/physiol Actions:	Four distinct colony-stimulating factors (CSFs) promoting survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte-macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF), macrophage-CSF (M-CSF), and interleukin-3 (IL-3, Multi-CSF). GM-CSF is a multipotential growth factor, stimulating proliferation of progenitor cells from more than one hematopoietic lineage (granulocyte, macrophage, and eosinophil). GM-CSF stimulates colony formation from pluripotent progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. GM-CSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It is produced by various cell types (activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to cytokine and inflammatory stimuli. In addition to granulocyte-macrophage progenitors, GMCSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF can induce nonhematopoietic cells such as endothelial cells to migrate and proliferate. It also stimulates the proliferation of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity GM-CSF receptors contain a GM-CSF-specific α?chain and a common α ?chain.
Physical form:	Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.
Preparation Note:	Recombinant Rat Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced from a DNA sequence encoding mature rat GM-CSF. This recombinant protein is a mixture of two rat GM-CSFs, the 127 amino acid form and the 128 amino acid methionyl form. Mature rat GM-CSF has a calculated mass of 14.7 kDa. GM-CSF, an acidic glycoprotein, is species-specific.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	>97% (SDS-PAGE)
Storage Temp.	−20°C
UNSPSC	12352202

Choose a favorite list for this item:

Catalog Number	Description	Price

		$

Please choose a list :

Continue Shopping

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.