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Glycerokinase from Bacillus stearothermophilus

SIGMA/G0774 - buffered aqueous solution, ≥75 units/mg protein (biuret)

Synonym: ATP:glycerol 3-phosphotransferase; Glycerol Kinase

CAS Number: 9030-66-4
EC Number: 232-862-0
MDL Number: MFCD00131205
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-G0774-500UN 500 units
$592.00
1/EA
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application(s) life science and biopharma
assay ≥5.0 mg protein/mL (biuret)
biological source Bacillus sp. (Bacillus steaarothermophilus)
color beige
form buffered aqueous solution
Quality Level 200 
specific activity ≥75 units/mg protein (biuret)
storage condition dry at room temperature
storage temp. 2-8°C
Application: Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.
Biochem/physiol Actions: Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.
General description: Research area: Cell Signaling

Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Other Notes: One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
Physical form: Stabilized solution in Tris buffer, pH 7.3
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥5.0 mg protein/mL (biuret)
activity specific activity: ≥75 units/mg protein (biuret)
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 2.7.1.30   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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