Glycopeptidase A from almonds
SIGMA/G0535 - buffered aqueous glycerol solution, ≥0.05 unit/mL
Synonym: N-Glycosidase A; N-
CAS Number: 83534-39-8
MDL Number: MFCD00131222
Product Type: Chemical
Figure 7. Cleavage site and structural requirements for Glycopeptidase A (PNGase A).
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
R1 = N- and C-substitution by groups other than H
R2 = H or the rest of an oligosaccharide structure
| concentration | ≥0.05 unit/mL |
| conjugate | (N-linked) |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| storage temp. | −20°C |
| Application: | Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp. |
| Biochem/physiol Actions: | Hydrolyzes an N4-(acetyl-β- |
| General description: | Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of ovalbumin glycopeptide per min at pH 5.0 at 37°C. |
| Physical form: | Solution in 50% glycerol containing 50 mM citrate-phosphate buffer, pH 5.0, and BSA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.5.1.52 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |