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β(1→4)-Galactosidase, positionally specific from Streptococcus pneumoniae

SIGMA/G0413 - recombinant, expressed in E. coli, buffered aqueous solution

CAS Number: 9031-11-2
MDL Number: MFCD01092684
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-G0413-1VL 1 vial
$676.00
1/EA
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form buffered aqueous solution
packaging vial of 0.06 unit
Quality Level 100 
recombinant expressed in E. coli
shipped in wet ice
specific activity ≥6 units/mg protein
storage temp. 2-8°C
UniProt accession no. P16278 
Application: β(1→4)-Galactosidase, positionally specific from Streptococcus pneumonia has been used:
• as a position-specific enzyme to study its effects in the terminal galactosylation with protective efficacy of glycosphingolipid (GSPL) in mice.
• for the digestion of radioactive oligosaccharides.
• as a position-specific enzymeto study its effects on the virulence profile of avirulent Leishmania donovani clone (A-LD).

Biochem/physiol Actions: β-Galactosidase plays a role in hydrolyzing the D-galactosyl moieties in oligosaccharides, polymers and secondary metabolites. It is widely applicable in the dairy industry to remove lactose from milk and dairy products for the benefit of lactose-intolerant individuals. β-Galactosidase is also applicable in the food industry to improve the sweetness, flavor and solubility.
General description: β-Galactosidase is present in bacteria, fungi, yeast and animal organs. It is also found in fruits, such as apples, almonds and apricots. β-Galactosidase is a tetramer and is made up of four polypeptide chains consisting of amino acids that assemble to form five structural domains. The domains are jelly roll barrel, a central domain that serves as an active site and the remaining domains are composed of β-sandwich and fibronectin.
Other Notes: One unit will hydrolyze 1 μmole of p-nitrophenyl β-D-galactopyranoside per min at pH 5.0 at 37 °C.
Physical form: Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: ≥6 units/mg protein
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.2.1.23   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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