Granulocyte colony-stimulating factor human
SIGMA/G0407 - >97% (SDS-PAGE), G-CSF, recombinant, expressed in E. coli, suitable for cell culture
MDL Number: MFCD00166475
Product Type: Chemical
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| assay | >97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | <0.10 EU/μg |
| mol wt | 18.8 kDa (175 amino acids including N-terminal methionine) |
| packaging | pkg of 5 and 25 μg |
| potency | 0.01-0.1 ng/mL |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P09919  |
| Analysis Note: | The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60. |
| Application: | Granulocyte colony-stimulating factor human has been used: • in culturing human neutrophils • as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34+ cells • to test its protective effects chronic liver damage model |
| Biochem/physiol Actions: | Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR). |
| Biochem/physiol Actions: | Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity. |
| General description: | Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle. |
| Physical form: | Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc, pH 3.5, containing 50 μg BSA per μg G-CSF |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |