Anti-GATA1 antibody produced in rabbit
SIGMA/G0290 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ERYF1; Anti-Erythroid transcription factor 1; Anti-Globin transcription factor 1
MDL Number: MFCD02096392
Product Type: Chemical
Western Blotting Whole extract of K562- Human erythroleukamia cells were separated on SDS-PAGE and probed with Anti-GATA1 antibody produced in Rabbit (Cat. No. G0290). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:400 antibody 2. 1:800 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 45 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:200 using a whole extract from a human chronic myelogenous leukemia cell line, K562 | |
| UniProt accession no. | P15976  |
| Application: | Anti-GATA1 antibody produced in rabbit has been used in gel mobility shift assay and DNase I footprinting. It has also been used in confocal microscopy analysis. |
| Biochem/physiol Actions: | GATA is a lineage-restricted transcription factor which plays a pivotal role in erythroid differentiation. It possesses potential binding sites in the regulatory sequences of all erythroid genes. GATA is expressed at higher levels during erythroid maturation. It is essential for terminal differentiation of erythrocytes and megakaryocytes and associated in vivo with the acetyl transferase p300/CBP. Mutation in GATA1 causes thrombocytopenia and abnormal platelet function. |
| Biochem/physiol Actions: | GATA-binding factor 1 (GATA-1) is involved in blocking apoptosis of precursor cells and in controlling the balance between proliferation and cell cycle arrest. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GATA is an important cis-element factor in the haematopoietic lineage belongs to GATA-binding protein family. It was first identified in the analysis of globin gene promoters and enhancers. There are different types of GATA proteins: GATA-1, GATA-2, GATA-3. It is expressed in erythroid, mast, megakaryocytic lineages, multipotential progenitor cells and lines. |
| General description: | GATA-1 (ERYF1, GF-1, NF-1) is a Cys2/Cys2 zinc finger DNA binding protein that is expressed primarily in erythroid, megakaryocytic, mast cells and eosinophilic cells. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of human GATA-1 (amino acids 394-413). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |