Granulocyte-Macrophage Colony-Stimulating Factor from mouse
SIGMA/G0282 - GM-CSF, from mouse, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: GM-CSF
MDL Number: MFCD00241918
Product Type: Chemical
SDS-PAGE Granulocyte-Macrophage Colony-Stimulating Factor from Mouse (Cat. No. G0282) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of GM-CSF that elicits a 50% attachment factor in cell based bioassay. Cell line: FDC-P1.
| assay | ≥97% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| form | lyophilized powder |
| impurities | ≤1 EU/mg |
| mol wt | ~15 kDa (125 amino acids including N-terminal methionine) |
| packaging | pkg of 5 and 50 μg |
| potency | ≤0.2 ng/mL EC50 (corresponds to ≥5 × 106 units/mg) |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble, clear, colorless |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01587  |
| Analysis Note: | The EC50 activity of mouse GM-CSF is tested in culture using murine FDP-1 cells. |
| Biochem/physiol Actions: | Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity. |
| Physical form: | Lyophilized from 10 mM acetic acid plus 250 μg BSA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |