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FLAG® Immunoprecipitation Kit

SIGMA/FLAGIPT1

Synonym: Anti-ddddk; Anti-dykddddk

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-FLAGIPT1-1KT 1 kit
 $1190.00
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Immunoblot Assay′s samples were assessed on SDS-PAGE and immunobloted with Anti-FLAG (Cat. No. F2555). Lanes: 1. Elution with X3 FLAG Peptide 2. Elution with Elution Buffer 3. Elution with Sample Buffer 4. Negative Control 5. Positive Control

 

analyte chemical class(es) proteins (FLAG® Affinity Gels)
Quality Level 300 
shipped in wet ice
storage temp. −20°C
technique(s) immunoprecipitation (IP): suitable
usage  kit sufficient for 50 reactions
Application: Learn more product details in our FLAG® application  portal.
Features and Benefits: • Utilizes highly specific ANTI-FLAG M2 affinity gel
• No preliminary steps or calibrations
• 3xFLAG™ peptide provides efficient and gentle elution through direct competition
General description: Sigma’s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Legal Information: 3xFLAG is a trademark of Sigma-Aldrich Co. LLC
Legal Information: FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: All prodedures to be performed at 2-8°C.
Storage Temp. −20°C
UNSPSC 12352200
Components ANTI-FLAG M2 affinity gel 1 mL; 3X FLAG peptide 1 mg

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