Fibrinogen from bovine plasma
SIGMA/F8630 - Type I-S, 65-85% protein (≥75% of protein is clottable)
Synonym: Factor I
CAS Number: 9001-32-5
EC Number: 232-598-6
MDL Number: MFCD00163624
Product Type: Chemical
Fibrinogen is synthesized in the liver and is circulated in plasma at ~200-450 mg/dL. It is a dimeric glycoprotein composed of three pairs of peptide chains. The two subunits are composed of a D domain which contains a globular region and an E domain which contains a disulfide bond that links the two subunits. Molecular Mass: Soluble Dimer: 340 kDa, α chain: 63.5 kDa, β chain: 56 kDa, γ chain: 47 kDa
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F8630-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F8630-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F8630-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F8630-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F8630-1G
| biological source | bovine plasma |
| concentration | 65-85% (biuret) |
| form | powder |
| mol wt | α-chain 63.5 kDa |
| β-chain 56 kDa | |
| γ chain 47 kDa (about 4% carbohydrate content) | |
| soluble dimer 340 kDa | |
| Quality Level | 300  |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| type | Type I-S |
| UniProt accession no. | P02672  |
| P02676 |
|
| P12799 |
| Analysis Note: | Protein determined by biuret |
| Application: | Fibrinogen has been used in studies of haemostatic therapy in surgical and massive trauma patients. These studies have shown that fibrinogen may prove to be more superior in stopping blood loss when compared to using fresh frozen plasma (FFP). |
| Biochem/physiol Actions: | Acute phase protein that is part of the coagulation cascade of proteins. |
| Biochem/physiol Actions: | Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyzes fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source. |
| Packaging: | 1, 5, 10, 25, 100 g in poly bottle |
| Physical form: | Contains ~10% sodium citrate and ~15% sodium chloride. |
| Preparation Note: | The optimal way to solubilize fibrinogen is to layer it on top of warm (37 ºC) saline, as fibrinogen will not dissolve in water. The saline concentration can be in the range of 0.85-0.9%. The fibrinogen-saline solution can be gently agitated, but it must not be vortexed. The fibrinogen will slowly dissolve to give a hazy solution. Fibrinogen may be sterile-filtered, but may not go through a 0.1 μm filter. A 0.2 μm filter is suggested, with positive pressure using a syringe and "button" filter. Vacuum filtration should not be used, since this will lead to breakdown of the molecule during the filtration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |