Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat
SIGMA/F8521 - affinity isolated antibody, buffered aqueous solution
MDL Number: MFCD00162622
Product Type: Chemical
Double immunofluorescent labeling PTK-1 cells in anaphase and interphase stained with Monoclonal and Anti-β-Tubulin (clone no. 2-28-33, Cat. No. T5293 and Goat Anti-Mouse IgG-FITC, F(ab′)2 fragment (Cat. No. F8521) and anti-centromere from human sera with Goat Anti-Human IgG-Cy3 (Cat. No. C2571). Chromosomes are counterstained with Hoechst (Cat. No. B2261). Images were acquired on a videomicroscope with a cooled CCD camera. From J. De Mey and P. Guillaud, Institute Jacques Monod, Paris.
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 |
| Application: | Anti-Mouse IgG (Fab specific) F(ab′)2 fragment−FITC antibody produced in goat has been used in: • flow cytometry for fluorescence-activated cell sorting (FACS) analysis • immunofluorescence and confocal microscopy • immunofluorescent HSP72 detection/immunocytochemi |
| Biochem/physiol Actions: | Binds all mouse Igs. Useful when trying to avoid background staining due to the presence of Fc receptors. Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody recognizes Fab fragment of mouse IgG. No cross reaction is observed with the Fc fragment of mouse IgG. No reactivity is observed with human, horse or bovine serum. The antibody is purified by affinity isolation and conjugated with FITC in an alkaline reaction. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system. |
| Biochem/physiol Actions: | Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. |
| General description: | Immunoglobulin G (IgG) is a member of the immunoglobulin family and contains gamma (γ) heavy chain in the constant (C) region. Monomeric structure of IgG contains two identical heavy chains and two identical light chains with molecular weight of 50 kDa and 25 kDa respectively. The primary structure of this antibody also contains disulfide bonds implicated in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3 and IgG4 with different heavy chains, named γ1, γ2, γ3 and γ4, respectively. |
| Immunogen: | Purified mouse IgG; The F(ab′)2 fragment of the antibody is obtained from pepsin digested antiserum |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with bovine, horse or human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |