Anti-Factor VII antibody, Mouse monoclonal
SIGMA/F8146 - clone HVII-1, purified from hybridoma cell culture
MDL Number: MFCD00164643
Product Type: Chemical
Western Blotting Human plasma were separated on SDS-PAGE and probed with Anti-Factor VII antibody, Mouse monoclonal, Clone: HVII-1 (Cat. No. F8146). The antibody was developed with 4 μg/mL Anti-Mouse IgG (Fab specific)-Biotin (Cat. No. B0529) followed by 1 μg/mL ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2636). Lanes 1. 1 μg/mL antibody 2. 2 μg/mL antibody 3. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HVII-1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1:4,000 | |
| UniProt accession no. | P08709  |
| Application: | Anti-Factor VII antibody, Mouse monoclonal has been used in: • the preparation of factor VII - depleted plasma and for purification • sandwich-type immunoassays • the evaluation of patients with hereditary factor VII deficiency by an enzyme linked immunosorbent assay (ELISA) or radio immunosorbent assay (RIA) • the assay of factor VII level in patients with liver disease (a sensitive parameter of liver dysfunction) • epidemiological studies of the importance of factor VII level as a risk factor for coronary heart disease, cerebrovascular disease and peripheral vascular diseases • the determination of the activity state of factor VII in in vivo samples when used with a clotting assay • flow cytometry |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Flow cytometry/Cell sorting (1 paper) |
| Application: | Monoclonal Anti-Factor VII antibody is suitable for flow cytometry analysis to study the behavior of TF-fVIIa secreted by ovarian cancer cells was associated with microparticles (MPs). It is also suitable for western blot at a dilution of 1:4,000 and indirect ELISA. |
| Biochem/physiol Actions: | Factor VII is a single-chain vitamin K-dependent glycoprotein in human coagulation. It has a molecular weight of ∼50,000Da, present in plasma in trace quantities. It is predominantly synthesized in liver and depends on the posttranslational γ−carboxylation of specific glutamic residues located near the NH2-terminus of the molecule. It is a serine protease zymogen and for activation it requires proteolytic cleavage in the disulfide-linked, two-chain form, Factor VIIa, by Factor Xa, Factor IXa, thrombin and Factor XIIa or β XIIa. |
| Biochem/physiol Actions: | The antibody recognizes an epitope on the light chain of Factor VII in the presence or absence of divalent cations. Clone no. HVII-1 (also cited as clone no. MC1476/E.A.8.1) may be used for purification of Factor VII and for the preparation of Factor VII depleted human plasma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Factor VII antibody, mouse monoclonal (mouse) IgG1 isotype) is derived from the HVII-1 hybridoma produced by the fusion of mouse Sp2/0-Ag14 myeloma cells and splenocytes from immunized BAB/c mice. Human coagulation factor VII contains 10 gamma-carboxyglutamic acid residues (Gla), located at the N-terminal region of the molecule. The activated factor VII (factor VIIa) also consists of a light chain with the Gla-domain and an epidermal growth factor domain, which is linked by a disulfide bond to a heavy chain, containing the serine protease catalytic domain. |
| Immunogen: | Factor VII from pooled normal human plasma. |
| Physical form: | Solution in 10 mM HEPES buffer, pH 7.4, with 140 mM sodium chloride and 0.05% sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |