Anti-FADD antibody, Mouse monoclonal
SIGMA/F8053 - clone FD19, purified from hybridoma cell culture
Synonym: Anti-Fas Associated Death Domain
MDL Number: MFCD01633337
Product Type: Chemical
Western Blotting Whole extract of A431 cells were separated on SDS-PAGE and probed with Anti-FADD antibody, Mouse monoclonal, Clone: FD19 (Cat. No. F8053). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1 μg/mL antibody 2. Negative Control
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FD19, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~28 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using total cell extracts of A431 cells | |
| UniProt accession no. | Q13158  |
| Application: | Anti-FADD antibody, Mouse monoclonal has been used in: • immunoblotting • immunoprecipitation • enzyme-linked immunosorbent assay (ELISA) |
| Biochem/physiol Actions: | FADD (Fas Associated Death Domain) is associated with various non-apoptotic cellular pathways such as regulation of cell cycle machinery in T lymphocytes connected to the phosphorylation state of FADD and to the FasL/TRAIL-induced transcriptional activation of c-fos protooncogene. It also interacts with the hepatitis C virus core protein in the HEK-293 cell line. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-FADD antibody, Mouse monoclonal (mouse IgG1) is derived from the FD19 hybridoma produced by the fusion of murine myeloma cells (NS1) and splenocytes from mouse immunized with purified recombinant human FADD. |
| Immunogen: | purified recombinant human FADD. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |