Anti-c-Fos antibody produced in rabbit
SIGMA/F7799 - IgG fraction of antiserum, buffered aqueous solution
Synonym: C-Fos Antibody; C-Fos Antibody - Anti-c-Fos antibody produced in rabbit
MDL Number: MFCD00239671
Product Type: Chemical
Immunofluorescence
Double staining with Rabbit Anti-cfos (Cat. No. F7799) (red) at a 1:5,000 dilution, and Monoclonal Anti-Calbindin-D-28K (Cat. No. C9848) (green) at a 1:400 dilution, reveals neuronal activation in a sub-population of lateral septal cells. Developed with biotinylated Donkey Anti-Rabbit, followed by Streptavidin-Cy3 (Cat. No. S6402) and FITC-labeled Goat Anti-Mouse.
From Dr. S. Shoham, Herzog Hospital Research Lab, Jerusalem, Israel.
Double staining with Rabbit Anti-cfos (Cat. No. F7799) (red) at a 1:5,000 dilution, and Monoclonal Anti-Calbindin-D-28K (Cat. No. C9848) (green) at a 1:400 dilution, reveals neuronal activation in a sub-population of lateral septal cells. Developed with biotinylated Donkey Anti-Rabbit, followed by Streptavidin-Cy3 (Cat. No. S6402) and FITC-labeled Goat Anti-Mouse.
From Dr. S. Shoham, Herzog Hospital Research Lab, Jerusalem, Israel.
Immunohistochemistry Formalin-fixed, paraffin-embedded human colon carcinoma sections stained with 1:5,000 Anti-c-Fos antibody produced in Rabbit (Cat. No. F7799) followed by Anti-Rabbit IgG-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting A431 nuclear extract were separated on SDS-PAGE and probed with Anti-c-Fos antibody produced in Rabbit (Cat. No. F7799). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 50-60 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using human colorectal carcinoma tissue |
| western blot: 1:1,000 using nuclear extract of a phorbol ester-treated human A431 cells or nuclear extract of human A431 cells | |
| UniProt accession no. | P01100  |
| Application: | Rabbit polyclonal anti-c-Fos antibody recognizes c-Fos by immunoblotting, (as single or multiple bands at 50-62 kDa) and by immunohistochemistry. By immunoblotting, the antibody may also detect bands of ~40 kDa representing c-Fos degradation products. Staining of c-Fos by immunoblotting is specifically inhibited with c-Fos immunizing peptide (c-Fos human, amino acids 3-16 with C-terminally added lysine). Anti-c-Fos antibody produced in rabbit has been used in immunoassays to determine the c-fos immunoreactivity in spinal cord sections. It has been used in Immunohistochemistry. It may be used as a probe to determine the presence and roles of c-Fos in a wide variety of cell processes including cell proliferation and differentiation, and responses to cell damage or stress. |
| Biochem/physiol Actions: | c-Fos (AP-1 transcription factor subunit) plays an important role in cell proliferation and differentiation. In addition, it is involved in cellular responses to stress, cell damage and death, and has a central role in normal bone and hematopoietic cell development and in oncogenesis. c-Fos is rapidly and transiently induced in almost every cell type upon stimulation by a variety of extracellular stimuli, including stress, mitogenic growth factors, cytokines, neurotransmitters and pharmacological agents. Increased expression of c-Fos is found in some human carcinomas, including colon and pancreatic adenocarcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | c-Fos belongs to a family of transcription factors, including FosB, Fra1, Fra2. The fos oncogene was isolated as a retroviral transforming gene (v-fos) carried by the FBJ-murine osteosarcoma virus. c-Fos undergoes post-translational modifications and other forms have been described including 57 Da, 60 kDa and 62 kDa proteins. Stable expression of c-Fos has been shown in developing bone tissue and teeth, hematopoietic cells,germ cells, and in the central nervous system. High levels of c-Fos are expressed in human full term fetal membranes. |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of the human c-Fos proto-oncogene (p55), amino acids 3-16. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |