Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal
SIGMA/F6162 - clone FB-8, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Fibroblast Growth Factor-Basic antibody produced in mouse; Anti-FGF2
MDL Number: MFCD00212948
Product Type: Chemical
Neutralizing ELISA The minimum titer of 5 μg/mL was determined by neutralization bioassay using recombinant human bFGF. Used at the recommended concentration, the antibody will neutralize the biological activity of 1 unit of bFGF on mouse fibroblast 3T3 cells.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FB-8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 18 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable |
| indirect ELISA: suitable | |
| neutralization: 5 μg/mL using 1 unit recombinant human FGF2 in mouse 3T3 cells | |
| radioimmunoassay: suitable | |
| western blot: suitable | |
| UniProt accession no. | P09038  |
| Application: | Anti-Fibroblast Growth Factor-Basic antibody has been used in in immunolabelling and western blotting. It may also be used in: • in immunoblotting • in enzyme linked immunosorbent assay (ELISA) • in dot blot • in radio immune assay (RIA) • in neutralization bioassays |
| Application: | Used in protein pin array assay to identify the interactive sequences in human αB crystalline protein. The antibody can be used for neutralization of the biological activity of FGF2 in cell culture. |
| Biochem/physiol Actions: | Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activating pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis. |
| Biochem/physiol Actions: | The antibody reacts specifically with both native and recombinant human FGF2. It does not cross-react with human FGF1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the FB-8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Fibroblast growth factors (FGFs) are members of a family of polypeptides. The names FGF acidic and basic are used for FGF-1 and FGF-2, respectively. Acidic and basic fibroblast growth factor (aFGF and bFGF, respectively) are members of a small family of heparin binding growth factors (HBGF). bFGF usually has an apparent mass of about 18 kDa. |
| Immunogen: | recombinant human FGF2. |
| Physical form: | Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |