Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse
SIGMA/F6140 - clone FN-3E2, ascites fluid
Synonym: Anti-CIG; Anti-ED-B; Anti-FINC; Anti-FN; Anti-FNZ; Anti-GFND; Anti-GFND2; Anti-LETS; Anti-MSF; Anti-SMDCF
MDL Number: MFCD00164562
Product Type: Chemical
Immunofluorescence Chicken fibroblast cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with1:400 Monoclonal Anti-Fibronectin, Cellular antibody produced in Mouse, clone: FN-3E2 (Cat. No. F6140). The antibody was developed using Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence Hs68 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:500 Anti-Cellular Fibronectin antibody, Mouse monoclonal, Clone: FN-3E2 (Cat. No. F6140). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Hs68 cells lysate was separated on SDS-PAGE and probed with Anti-Cellular Fibronectin antibody, Mouse monoclonal, Clone: FN-3E2 (Cat. No. F6140). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Negative control: without first antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FN-3E2, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| mol wt | antigen 240 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: 1:400 using cultured chicken fibroblasts | |
| radioimmunoassay: suitable | |
| western blot: suitable | |
| UniProt accession no. | P02751  |
| Application: | Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in: • immunoblot • immunofluorescent microscopy • immunocytochemistry |
| Biochem/physiol Actions: | Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis . |
| Biochem/physiol Actions: | Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin. |
| Biochem/physiol Actions: | The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc. |
| Immunogen: | fibronectin antigen released in culture by a human breast cancer cell line |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |