Anti-Horse Spleen Ferritin antibody produced in rabbit
SIGMA/F6136 - affinity isolated antibody, lyophilized powder
Synonym: Ferritin Antibody
MDL Number: MFCD00162386
Product Type: Chemical
ELISA Anti-Horse Spleen Ferritin antibody produced in Rabbit (Cat. No. F6136), followed by 1:8,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Horse Spleen Ferritin by indirect ELISA.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| species reactivity | horse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoelectrophoresis: suitable |
| indirect ELISA: 1:10,000 |
| Application: | Anti-Horse Spleen Ferritin antibody was used for immunocytochemistry of rat brain sections at a working antibody dilution of 1:2000 and in mouse brain sections at 1:1000. The antibody was used for immunoblotting using cell lysates of mouse primary erythroblasts and human ovarian carcinoma cells and human bronchial epithelial cells at a working dilution of 1:1000. The antibody is suitable for immunoelectrophoresis. |
| Application: | Horse Spleen Ferritin antibody has been used: • immunofluorescence • in western blotting • in immunohistochemistry |
| Biochem/physiol Actions: | Ferritin iron is oxidized from Fe(II) to Fe(III) in the presence of the enzyme ferroxidase. |
| Biochem/physiol Actions: | The antibody specifically recognizes ferritin from horse spleen. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ferritin consists of homogenous protein segment called as apoprotein and an iron segment which binds to the apoprotein. It is composed of heavy (H) and light (L) chains. |
| General description: | Ferritin is the storage form of iron in mammals, found mainly in liver, bone marrow and spleen. It is also present in small intestine, placenta, kidney and heart. Horse spleen ferritin is made up of 24 identical subunits. Ferritin of different organs is distinct with different electrophoretic mobility and possible functional differences. . |
| Immunogen: | Purified horse spleen ferritin |
| Physical form: | Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2 |
| Preparation Note: | Reconstitute with 0.135 M sodium chloride. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |