Ficin from fig tree latex
SIGMA/F6008 - lyophilized powder
Synonym: Debricin; Ficain; higueroxyl delabarre
CAS Number: 9001-33-6
EC Number: 232-599-1
MDL Number: MFCD00081597
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F6008-100UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F6008-200UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F6008-1KU
| biological source | fig tree (latex) |
| composition | Protein, 80-100% biuret |
| form | lyophilized powder |
| mol wt | 23.8 kDa |
| Quality Level | 300  |
| specific activity | ≥1.0 units/mg protein |
| storage temp. | −20°C |
| Application: | Ficin from fig tree latex has been used: • in the proteolysis of tendon collagen • in the digestion of Chlorophyta Ulva rigida • to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin |
| Biochem/physiol Actions: | Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanili |
| Biochem/physiol Actions: | Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure. |
| General description: | Extinction Coefficient: E1% = 21.0 (280 nm) pI: 9.0 |
| Other Notes: | One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path). |
| Packaging: | 100, 200, 1000 units in poly bottle |
| Physical form: | Lyophilized powder containing |
| Preparation Note: | This enzyme is soluble in water (10 mg/ml), yielding a clear solution. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| activity | specific activity: ≥1.0 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.22.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |