Anti-Mouse IgG (Fc specific)–FITC antibody produced in goat
SIGMA/F5897 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fc specific)
MDL Number: MFCD00162628
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547), followed by 1:200 Anti-Mouse IgG (Fc specific)-FITC antibody produced in Goat (Cat. No. F5897).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:50 Monoclonal Anti-β 2-Microglobulin antibody produced in Mouse, Clone: BM-63 (Cat. No. M7398) followed by 1:64 Anti-Mouse IgG (Fc specific)-FITC antibody produced in Goat (Cat. No. F5897).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 |
| indirect immunofluorescence: 1:64 |
| Application: | Anti-Mouse IgG (Fc specific)−FITC antibody produced in goat has been used in: • immunohistochemistry • immunofluorescence • anti-nuclear antibody assay |
| Biochem/physiol Actions: | Anti-Mouse IgG (Fc specific)-FITC antibody is specific for Fc fragment of all mouse IgG subclasses. It shows no reactivity with mouse IgG Fab fragment, rat IgG or human IgG. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. |
| Biochem/physiol Actions: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. |
| Immunogen: | Purified mouse IgG |
| Other Notes: | Antibody adsorbed with human IgG and rat serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with human or rat samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |