o-Dianisidine dihydrochloride
SIGMA/F5803 - Suitable for use in glucose determination
Synonym: 3,3′-Dimethoxybenzidine dihydrochloride; Fast Blue B
CAS Number: 20325-40-0
Empirical Formula (Hill Notation): C14H16N2O2 · 2HCl
Molecular Weight: 317.21
EC Number: 243-737-5
MDL Number: MFCD00012488
Linear Formula: C14H16N2O2 · 2HCl
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| form | crystalline |
| InChI | 1S/C14H16N2O2.2ClH/c1-17- |
| InChI key | UXTIAFYTYOEQHV-UHFFFAOYSA |
| mp | 268 °C |
| pH range | 6.95-7.05 |
| Quality Level | 200  |
| SMILES string | Cl.Cl.COc1cc(ccc1N)-c2ccc |
| solubility | water: 2.5 mg/mL, colorless |
| storage temp. | 2-8°C |
| Application: | o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies: • As substrate for the determination of serum ceruloplasmin from its oxidase activity. • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method. • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD). • Myeloperoxidase activity assay. |
| Biochem/physiol Actions: | o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl. |
| Disclaimer: | Probably carcinogenic. |
| General description: | A preweighed vial containing 50 mg o-Dianisidine. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H350 |
| Precautionary statements | P201 - P301 + P312 + P330 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 45-22 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 268 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12171500 |