Monoclonal Anti-Folic Acid antibody produced in mouse
SIGMA/F5766 - clone VP-52, ascites fluid
MDL Number: MFCD00162295
Product Type: Chemical
ELISA Monoclonal Anti-Folic Acid antibody produced in Mouse, Clone: VP-52 (Cat. No. F5766), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Folic Acid by indirect ELISA. Reactivity is observed only with Folic Acid and 5-MTH (Folic Acid)-BSA but not with Vitamin B12-BSA and BSA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | VP-52, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2b |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:500 |
| Application: | Monoclonal Anti-Folic Acid antibody is suitable for use in western blot (diluted 1:1000). |
| Biochem/physiol Actions: | Folic acid (folate) is a vitamin that regulates one-carbon transfer during the conversion of homocysteine to methionine. Methionine is a precursor of S-adenosylmethionine, which is the main methyl donor for methylation reactions. Thus, folic acid is essential for regulation of DNA methylations and for epigenetic modifications that modulate gene expression. Folate deficiency has been associated with a wide range of disorders including colorectal cancers, depression, neural tube defects and cerebral dysfunctions |
| Biochem/physiol Actions: | Folic acid is essential for normal growth of mammalian cells. Folic acid deficiencies are common in pregnant women, alcoholics, those whose diets do not include raw fruits and vegetables, and people with structural or functional damage to the small intestine. Vitamin B12 and folic acid are metabolically interrelated. |
| Biochem/physiol Actions: | Monoclonal Anti-Folic Acid antibody binds specifically to folate and detects an epitope present on both folic acid and its biologically active analog 5-methyltetrahydrofolic acid (5MTHFA). The product reacts with free folate, folate conjugated to a carrier such as KLH or BSA, and folic acid in human plasma or serum bound to the endogenous Folate Binder. The antibody does not cross-react with tetrahydrofolic acid, folinic acid or dihydrofolic acid. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Folic Acid (mouse IgG2b isotype) is derived from the hybridoma produced from the fusion of mouse myeloma cells and splenocytes of an immunized mouse. The normal range for folate in serum is 3-13 ng/ml. |
| Immunogen: | 5-Methyltetrahydrofolic acid (5MTHFA) conjugated to KLH |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |