Monoclonal Anti-FITC antibody produced in mouse
SIGMA/F5636 - clone FL-D6, ascites fluid
Synonym: Monoclonal Anti-FITC
Product Type: Chemical
ELISA Monoclonal Anti-FITC antibody produced in Mouse, Clone: FL-D6 (Cat. No. F5636), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect FITC by indirect ELISA. Reactivity is observed with FITC-ExtrAvidin® but not with TRITC-ExtrAvidin®.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | FL-D6, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:2,500 using FITC-conjugate |
| indirect immunofluorescence: suitable |
| Application: | Mouse monoclonal clone FL-D6 anti-FITC antibody may be used for the detection of FITC and as a universal indicator reagent for bridging FITC with other immunochemical reagents. It may be used in ELISA, competitive ELISA and immunofluorescent techniques. A FITC Anti-FITC system has been used in the amplification of signal in immunofluorescent detection and as a means of separating bound from free tracer by affinity chromatography. The antibody can also be used to isolate cells that have an FITC-labeled ligand on their surface. |
| Biochem/physiol Actions: | Mouse monoclonal clone FL-D6 anti-FITC antibody will react with either free or conjugated FITC. The antibody does not react with bound or free TRITC (tetramethylrhodamine isothiocyanate). |
| Biochem/physiol Actions: | The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques because the conjugation of FITC to proteins is relatively easy and generally does not destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Antibodies to FITC are used to identify FITC labeled proteins and as models to study the mechanism of antibody response to a well defined hapten. |
| Immunogen: | FITC-BSA conjugate |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |