Ficin from fig tree latex
SIGMA/F4125 - saline suspension, ≥1.0 units/mg protein (biuret)
Synonym: Debricin; Ficain; higueroxyl delabarre
CAS Number: 9001-33-6
MDL Number: MFCD00081597
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| biological source | fig tree (latex) |
| form | saline suspension |
| mol wt | 23.8 kDa |
| Quality Level | 200  |
| specific activity | ≥1.0 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| Application: | Ficin from fig tree latex has been used: • in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality • for the digestion of eye tissue sections prior to immunostaining and immunofluorescence |
| Biochem/physiol Actions: | Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanili |
| General description: | Extinction Coefficient: E1% = 21.0 (280 nm) pI: 9.0 |
| General description: | Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges. |
| Other Notes: | One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path). |
| Packaging: | 1000 units in glass bottle |
| Physical form: | Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0 |
| Preparation Note: | 2× Crystallized |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 23-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥1.0 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.22.3 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |