Anti-FMR1 (C-terminal) antibody produced in rabbit
SIGMA/F4055 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-FMRP; Anti-Fragile X Mental Retardation Protein
Product Type: Chemical
Immunofluorescence
Localization of FMR1 in cytoplasmic stress like granules in NIH-3T3 cells. Cells were heat shocked for 30 min. at 43 ºC, fixed and stained with 2 μg/mL Rabbit Anti-FMR1 (C-terminal) (Cat. No. F4055). A. 100× magnification; B. 40× magnification.
Localization of FMR1 in cytoplasmic stress like granules in NIH-3T3 cells. Cells were heat shocked for 30 min. at 43 ºC, fixed and stained with 2 μg/mL Rabbit Anti-FMR1 (C-terminal) (Cat. No. F4055). A. 100× magnification; B. 40× magnification.
Enhanced Validation-By Functional Assay Immunofluorescence. Antibody recognition: Anti-FMR1 (C-terminal) antibody produced in Rabbit (Cat. No. F4055) is confirmed by immunofluorescence in the NIH3T3 cell sample that has been treated with heat shock (Picture B) while no spefic staining is detected in the untreated sample-NIH3T3 (Picture A). Cells were counterstained with DAPI (blue) to stain nuclei. Antibody dilution 5 μg/mL.
Immunoprecipitation Rabbit Anti-FMR1 (C-terminal) (Cat. No. F4055) was used to immunoprecipitate human FMR1 from HEK-293T cell lysates. Lanes 1. 10 μg antibody. Detection antibody: Rabbit Anti-FMR1 (N-terminal) (Cat. No. F3930) 2-4. 10 μg, 5 μg and 3 μg antibody, respectively. Detection antibody FMR1 (C-terminal) (Cat. No. F4055) 5. Negative control – without cell lysate 6. Negative control – without IP antibody 7. Positive control – direct immunoblotting of HEK-293T cell extract
Immunoblotting
Lysates from various cell lines were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-FMR1 (C-terminal) (Cat. No. F4055). The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lysates from the following cell lines were loaded:
Lanes: 1. HEK-293T; 2. HeLa; 3. A431; 4. RINSH; 5. RAT1; 6. PC12; 7. C2.
Lysates from various cell lines were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-FMR1 (C-terminal) (Cat. No. F4055). The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lysates from the following cell lines were loaded:
Lanes: 1. HEK-293T; 2. HeLa; 3. A431; 4. RINSH; 5. RAT1; 6. PC12; 7. C2.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| enhanced validation | functional assay Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~80 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using HEK-293T cells lysate |
| indirect immunofluorescence: 2-5 μg/mL using methanol-acetone fixed heat-shocked NIH3T3 cells | |
| western blot: 1-2 μg/mL using HEK-293T cell lysate | |
| western blot: 2-4 μg/mL using RAT1 cell lysate | |
| UniProt accession no. | Q06787  |
| Application: | Anti-FMR1 (C-terminal) antibody produced in rabbit has been used in: western blotting, immunoprecipitation, immunofluorescence, immunoblotting . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | FMR1 is a RNA binding protein expressed mainly in brain, neurons, placenta, testes and lymphocytes. Defect in FMR1 can lead to Fragile X Mental Retardation Syndrome due to lack of expression of FMR1 or expression of a mutant protein that cannot bind RNA. Anti-FMR1 (C-terminal) antibody can be used in immunofluorescence staining. Rabbit anti-FMR1 (C-terminal) antibody reacts specifically with FMR1. |
| General description: | The FMR1 protein (fragile X mental retardation 1 protein) can bind to RNA. It contains two heterogeneous nuclear ribonucleoprotein K homology (KH) domains and one RGG box. Two proteins named FXR1 and FXR2 interact with FMR1. The protein is highly expressed in brain and testis. |
| Immunogen: | synthetic peptide corresponding to amino acids 606-623 of human FMR1, conjugated to KLH. The corresponding sequence is highly conserved (1 amino acid substitution) in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |