Proteasome Fraction II from rabbit
SIGMA/F4051 - 5 mg/mL, Reticulocyte cell extract, solution
MDL Number: MFCD03458197
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| concentration | 5 mg/mL |
| form | solution |
| Quality Level | 200  |
| storage temp. | −70°C |
| Application: | Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml. |
| Biochem/physiol Actions: | Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms. |
| Biochem/physiol Actions: | Protein fraction of a cell extract that binds to anion-exchange resin. |
| General description: | Manufactured for Sigma by Boston Biochem., Inc. |
| Physical form: | Solution in 25 mM HEPES, pH 7.6. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |