ANTI-FLAG® M2-FITC antibody, Mouse monoclonal
SIGMA/F4049 - clone M2, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal ANTI-FLAG® M2; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
Immunofluorescence
COS 7 cells transfected with pFLAG-CMV2-BAP plasmid stained with ANTI-FLAG M2 FITC Conjugate (Cat No. F4049).
COS 7 cells transfected with pFLAG-CMV2-BAP plasmid stained with ANTI-FLAG M2 FITC Conjugate (Cat No. F4049).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M2, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone |
| Application: | Antibody is suitable for immunofluorescence, immunocytochemistry, immunohistochemistry, immunoblotting, flow cytometry and electron microscopy. Ideal for use when detection of Flag fusion proteins is required in a murine host, where secondary antibodies could cause cross reactivity. Learn more product details in ourFLAG® application portal. |
| General description: | Anti Flag M2-FITC antibody is used for the detection of Flag fusion proteins.This monoclonal antibody is covalently conjugated to flourescein isothiocyanate (FITC). The antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.Binding is not Calcium dependent. F4049 is an Affinity purified antibody, increasing sensitivity in most applications. |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Packaging: | 0.2, 1, 5×1 mg in poly bottle |
| Physical form: | Solution in 10 mM phosphate buffered saline, plus 1% BSA and 0.1% sodium azide, pH 7.4 |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |