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Monoclonal ANTI-FLAG® M5 antibody produced in mouse

SIGMA/F4042 - clone M5, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-ddddk; Anti-dykddddk

MDL Number: MFCD00282246
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-F4042-.2MG 0.2 mg
 $543.00
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45-F4042-1MG 1 mg
 $1230.00
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45-F4042-5MG 5 mg
 $2050.00
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antibody form purified immunoglobulin
antibody product type primary antibodies
biological source mouse
clone M5, monoclonal
concentration 2-5 mg/mL
conjugate unconjugated
form buffered aqueous solution
immunogen sequence DYKDDDDK
isotype IgG1
purified by using Protein A
Quality Level 200 
shipped in dry ice
species reactivity all
storage temp. −20°C
technique(s) western blot (chemiluminescent): 10 μg/mL
Application: Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Application: Monoclonal ANTI-FLAG® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.
General description: Monoclonal ANTI-FLAG® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A
Immunogen: FLAG; peptide sequence DYKDDDDK
Legal Information: ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information: FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Antibody is not calcium dependent.
Physical form: Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Preparation Note: Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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