Anti-Mouse IgG (Fab specific)−FITC antibody produced in goat
SIGMA/F4018 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fab specific)
MDL Number: MFCD00162622
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human appendix tissue sections were stained with 1:400 Monoclonal Anti-Actin, α-Smooth Muscle, Clone: 1A4 (Cat. No. A2547) followed by 1:400 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F4018).
Immunofluorescence Human Periferal Blood Lymphocytes probed and developed using 1:100 Monoclonal Anti-β2-Microglobulin antibody produced in Mouse, Clone: BM-63 (Cat. No. M7398) followed by 1:64 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F4018).
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 |
| indirect immunofluorescence: 1:64 |
| Application: | Anti-Mouse IgG (Fab specific) FITC antibody produced in goat has been used in : • fluorescent staining • immunocytochemistry • immunofuorescence |
| Biochem/physiol Actions: | Anti-Mouse IgG (Fab specific)-FITC antibody is specific for Fab fragment of all mouse IgG subclasses. Goat anti-Mouse IgG is conjugated to FITC and then purified by gel filtration to remove free FITC. The antibody is adsorbed with human IgG to ensure minimal cross reactivity. The conjugate shows no reactivity with mouse IgG, Fc fragment, human, bovine, or horse serum. |
| Biochem/physiol Actions: | IgG (immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG (immunoglobulin G) is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure. |
| General description: | IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. |
| Immunogen: | Purified mouse IgG Fab fragment |
| Other Notes: | Antibody adsorbed with bovine, equine and human serum proteins. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | Adsorbed to reduce background staining with bovine, horse, or human samples. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |