Anti-Dog IgG (whole molecule)–FITC antibody produced in rabbit
SIGMA/F4012 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Rabbit Anti-Dog IgG (whole molecule)
MDL Number: MFCD00221535
Product Type: Chemical
Immunofluorescence Dog periferal blood lymphoctes were stained with 1:32 Anti-Dog IgG (whole molecule)-FITC antibody produced in rabbit (Cat. No. F4012).
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:32 |
| Application: | Anti-Dog IgG (whole molecule)-FITC antibody has been used in indirect fluorescent antibody test(IFAT) |
| Application: | Rabbit polyclonal anti-Dog IgG (whole molecule)–FITC antibody may be used as a secondary antibody or to detect the presence of dog IgG via fluorogenic immunochemical or immunohistochemical techniques. |
| Biochem/physiol Actions: | IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495nm and emits at 521nm. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG is present in large quantities in the human serum and about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bon |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |