Anti-Actin, α-Smooth Muscle - FITC antibody, Mouse monoclonal
SIGMA/F3777 - clone 1A4, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Actin, α-Smooth Muscle; alpha SMA-FITC antibody; alpha smooth muscle actin - FITC antibody; SMA
Product Type: Chemical
Western Blotting JOURNAL CITATION: Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. By: Yao, Y., Chen, Z. L., et al. in Nat Commun, 2014. PubMed ID: 24583950 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/ncomms4413), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. By: Yao, Y., Chen, Z. L., et al. in Nat Commun, 2014. PubMed ID: 24583950 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/ncomms4413), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity. By: Yao, Y., Chen, Z. L., et al. in Nat Commun, 2014. PubMed ID: 24583950 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/ncomms4413), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human appendix stained with 1:500 FITC-Anti-Actin α-smooth muscle antibody, Mouse monoclonal, clone: 1a4 (Cat. No. F3777).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A4, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, chicken, frog, canine, guinea pig, rabbit, bovine, goat, sheep, snake |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human tonsil or appendix |
| UniProt accession no. | P62736  |
| Application: | Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees. |
| Application: | Monoclonal Anti-Actin, α-Smooth Muscle - FITC antibody is suitable for: • immunohistochemical (IHC) staining of heart, lung, kidney, and liver of mice to examine the PHD2 deficiency in internal organs • immunostaining to analyze vascular smooth muscle cells/pericytes development in platelet-derived growth factor (PDGFR) and PDGFR- β-/- embryos • immunofluorescent analysis of implanted cells, frozen tissue samples to study the similar levels of expression of SMaA and SM-MHC as established primary SMC lines • western blots of lens epithelial cells to determine the distribution of α-SMA expression in lens epithelial cell • Immunohistochemical examination of lesion morphology • flow cytometry • immunohistochemistry, proliferation and TUNEL (terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling) assays • immunocytochemistry |
| Application: | Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin. IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts. |
| Biochem/physiol Actions: | Actin plays an important role in cell motility, structure and integrity. Smooth muscle actin-α (SMA)/ α2-smooth muscle actin (ACTA2) interacts with β-myosin heavy chain and helps in vascular smooth muscle cell contraction. The encoded protein modulates the expression of c-MET (tyrosine-protein kinase Met) and focal adhesion kinase (FAK) in human lung adenocarcinoma cells, which positively and selectively mediates tumor progression. Mutation in the gene expression leads to thoracic aortic aneurysms and dissections (TAAD). |
| Biochem/physiol Actions: | Monoclonal Anti-Actin, α-Smooth Muscle specifically recognizes the α-smooth muscle isoform of actin (42 kDa) by ELISA and immunoblotting. It does not react with the other major actin isoforms present in fibroblasts or epithelial cells (β and γ-cytoplasmic), striated muscle (α-sarcomeric), myocardium (α-myocardial), or γ-smooth muscle isoform. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | α-smooth muscle actin, a smooth muscle cell-specific protein, is also known as aortic smooth muscle or a smooth muscle actin (α-SMA, SMactin, α-SM-actin, ASMA). It belongs to the actin family.It is predominantly found in cells of the smooth muscle lineages. It has been reported that α-smooth muscle actin was also found in certain non-muscle cells, in particular fibroblasts i.e. myofibroblasts. Actin, a conserved eukaryotic protein, is the major cytoskeletal component and is expressed as six different isoforms in mammals and birds. It is mapped to human chromosome 10q23.31. The antibody reacts with normal and neoplastic, human vascular and visceral smooth muscle cells. It reacts with normal myoepithelial cells, pericytes, eye lens cells, hair follicle cells and certain stromal cells in the intestine, testis, lymphoid organs, liver, ovary and bone marrow. The antibody also reacts with stromal myofibroblasts in hypertrophic scars, and in neoplastic tissues. α-smooth muscle actin is transiently co-expressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It was also found in the ventricular conducting tract of adult mammalian heart. |
| General description: | The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. |
| Immunogen: | N-terminal synthetic decapeptide of α-smooth muscle actin. |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Protect from prolonged exposure to light. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |