Fibroblast Growth Factor-Basic
SIGMA/F3685 - FGF-Basic, from human, recombinant, expressed in E. coli, carrier free
Synonym: FGB-b; FGF-2; HBGF-2; HBGH-2; Prostatropin; bFGF
Product Type: Chemical
SDS-PAGE Fibroblast Growth Factor-Basic (Cat. No. F3685) was assessed on SDS-PAGE.
Bioassay Basic growth factor is a protein that is mitogenic for a variety of cell types. BFGF growth factor potency is quantified by its ability to stimulate a growth of Balb/3T3 cells in serum free conditions. The relative cell growth is detected using a fluorimetric assay based on the measurement of the conversion of Resazurin to Resorufin by metabolically active cells. The amount of BFGF that will give 50% of the maximal proliferation (EC50) is calculated from the different amounts assayed.
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| color | white to faint yellow cast |
| form | lyophilized powder |
| mol wt | 16.0 kDa |
| packaging | pkg of 25 μg |
| pkg of 4X25 μg | |
| potency | <1 ng/mL Biological Activity EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 0.025 mg, clear, colorless to faintly yellow |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P09038  |
| General description: | Since FGF-Basic is found in a variety of organs, acts on a wide range of cell types, and has multifunctional actions, it has acquired numerous synonyms, including heparin-binding growth factor (class II or beta), eye-derived growth factor I, cartilage-derived growth factor, and astroglial growth factor II5. Purified bovine and human FGF-basic differ by 3 amino acids in sequence3, and are biologically and immunologically cross-reactive. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |