Fluorescein 5(6)-isothiocyanate

SIGMA/F3651 - ≥90% (HPLC)

Synonym: FITC; Fluorescein isothiocyanate

CAS Number: 27072-45-3
Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
Molecular Weight: 389.38
EC Number: 248-207-7
MDL Number: MFCD00005063
Linear Formula: C₂₁H₁₁NO₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-F3651-100MG	100 mg	$118.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing hematology histology
assay	≥90% (HPLC)
color	yellow to orange
form	powder
InChI	1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key	MHMNJMPURVTYEJ-UHFFFAOYSA-N
Quality Level	200
SMILES string	Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
solubility	acetone: 1 mg/mL, clear, light yellow to yellow
storage temp.	2-8°C
technique(s)	titration: suitable

Application:	Fluorescein 5(6)-isothiocyanate has been used: • for fluorescence labeling of bacteria • as a standard for calibration of fluorometer • for the labeling of exosomes
Application:	For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography
General description:	Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Packaging:	100 mg in glass bottle

Hazard Codes	Xn
Risk Statements	42
Safety Statements	22-24/25
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3

Purity	≥90% (HPLC)
Storage Temp.	2-8°C
UNSPSC	12171500

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