Anti-Fibronectin antibody produced in rabbit
SIGMA/F3648 - affinity isolated antibody, buffered aqueous solution
Synonym: Fibronectin Antibody; Fibronectin Antibody - Anti-Fibronectin antibody produced in rabbit; Fibronectin Antibody Sigma
MDL Number: MFCD00162291
Product Type: Chemical
Western Blotting JOURNAL CITATION: Py2T murine breast cancer cells, a versatile model of TGFβ:-induced EMT in vitro and in vivo. By: Waldmeier, L., Meyer-Schaller, N., et al. in PLoS One, 2012. PubMed ID: 23144919 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0048651), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Role of vegetation-associated protease activity in valve destruction in human infective endocarditis. By: Al-Salih, G., Al-Attar, N., et al. in PLoS One, 2012. PubMed ID: 23029186 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0045695), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunocytochemistry-immunoflourescence JOURNAL CITATION: Distinct types of fibrocyte can differentiate from mononuclear cells in the presence and absence of serum. By: Curnow, S. J., Fairclough, M., et al. in PLoS One, 2010. PubMed ID: 20305780 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0009730), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence human HS-68 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 1:400 Rabbit Anti-Fibronectin (Cat. No. F3648). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000 using human fibronectin |
| indirect immunofluorescence: 1:400 using human foreskin cultured fibroblasts | |
| microarray: suitable | |
| western blot: 1:1,000 using human plasma fibronectin | |
| UniProt accession no. | P02751  |
| Application: | Anti-Fibronectin antibody is suitable for • immunohistochemistry (IHC) analysis to study the activity of pancreatic stellate cells in chronic pancreatitis • antibody blocking assays of bacterial cells to study the activity of fibronectin in the Yersinia pestis binding to host cells and Yop delivery • staining of endothelial cells as primary antibody • cell adhesion assays to study the effect of anti-human fibronectin antibodies on adhesion to endothelial cells • immunoprecipitation and western blot analysis of NF-κB dependent mammary tumorigenesis study • immunofluorescence staining to study the correlation between zyxin tails and fibrillar adhesions • immunoblotting to study the expression levels of the pancreatic stellate cells (PSC) markers glial fibrillary acidic protein (GFAP), α-smooth muscle actin (α-SMA), collagen type I, and fibronectin in whole cell lysates • ELISA of fibronectin secretion • microarray analysis. |
| Biochem/physiol Actions: | Fibronectin (FN) plays important roles in cell adhesion, migration, growth and differentiation. It has capacity to bind various functionally important domains such as heparin, collagen/gelatin, and fibrin. It also stimulates human non-small cell lung carcinoma. Numerous studies have shown that fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. Moreover, it has been shown that upon malignant transformation many cells lose most of their surface bound fibronectin. Fibronectin has been shown to also play a role in cellular morphology, cytoskeletal organization, phagocytosis, hemostasis, embryonic differentiation and wound repair. Fibronectin is produced by a wide variety of epithelial and mesenchymal cells in vitro including: fibroblasts, chondrocytes, myoblasts, Schwann cells, macrophages, hepatocytes and intestinal epithelial cells. Cellular fibronectin is present in many tissues including spleen, lymph node, tonsil, blood vessel walls, liver, kidney, muscle, skin, brain and peripheral nerves. It is found in basement membranes and in loose connective tissue stroma. It is also present in platelet α-granules and is expressed on the platelet surface after activation. |
| Biochem/physiol Actions: | The product shows no cross-reaction with laminin, vitronectin, collagen type IV, or chondroitin sulfate types A, B and C using a dot blot immunoassay. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Antiserum is produced in rabbit using purified human fibronectin as the immunogen. Affinity isolated antibody is obtained from antiserum by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to human fibronectin. The antiserum is determined to be immunospecific for human fibronectin by immunofluorescent labeling of human fibroblast cell cultures, ELISA and immunoblotting. In immunoblotting, a specific band of fibronectin at 220 kDa is observed (another band at 94 kDa may be also be present) using human fibronectin. When used in immunoelectrophoresis, the antibody shows 1-2 arcs of precipitation versus normal human plasma. This product may be used for immunohistochemical localization of fibronectin in normal, inflamed and neoplastic tissues, for detection of fibronectin on cultured cells and structure and function studies of fibronectins in human and animal body fluids, tissues and cells. Affinity isolated antibody to human fibronectin can be used for immunofluorescent and immunoperoxidase staining of cultured cells, frozen sections and formalin fixed, paraffin-embedded tissues. Other fixatives, e.g. methacarn and ethanol, may also be used. Fibronectin (FN) is composed of two nearly identical disulfide-bound polypeptides of molecular weight 220 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma, therefore polyclonal antibodies to either form usually cross-react. Careful analysis of the fibronectin molecule indicates that it contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, fibrinogen or fibrin, complement, glycosaminoglycans, proteoglycans and heparin. Fibronectin 1 is encoded by the gene mapped to human chromosome 2q35. |
| General description: | Fibronectin (FN) is a multifunctional, extracellular matrix glycoprotein. Cellular fibronectin exists as dimeric or cross-linked multimeric forms, made up of fibroblasts, epithelial and other cell types. The dimer form composed of two nearly identical ~250 kDa subunits linked covalently to the C-termini by a pair of disulfide bonds. FN is present as dimeric form in plasma and secreted by hepatocytes. |
| Immunogen: | Fibronectin isolated from human plasma |
| Physical form: | Solution in 0.01M phosphate buffered saline, pH 7.4 containing 1% BSA and 15 mM sodium azide |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |