Anti-von Willebrand Factor antibody produced in rabbit
SIGMA/F3520 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Von Willebrand Factor Antibody; Von Willebrand Factor Antibody - Anti-von Willebrand Factor antibody produced in rabbit; Anti-Factor VIII-Related Antigen
MDL Number: MFCD00162556
Product Type: Chemical
Immunofluorescence Equine exuberant granulation (proud flesh) tissue stained using Anti-von Willebrand Factor (Cat. No. F3520) at a 1:100 dilution and Anti-Rabbit IgG-FITC (Cat. No. F0382) (green) and DAPI (blue). From Lynn Burgess, Natural Sciences Department, Dickinson State University, Dickinson, ND.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor (Cat. No. F3520) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Human Kidney sections with 1:2,000 Rabbit Anti-von Willebrand Factor (Cat. No. F3520) using biotin/ExtrAvidin®-Peroxidase.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| packaging | antibody small pack of 25 μL |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling) |
| indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue | |
| UniProt accession no. | P04275  |
| Application: | Anti-von Willebrand Factor antibody produced in rabbit has been used for: • western blot analysis and immunostaining • immunocytochemistry • immunohistochemistry • indirect immunofluorescence |
| Biochem/physiol Actions: | It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII. |
| Biochem/physiol Actions: | The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin′s or ethanol-fixed and paraffin-embedded tissue sections. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-von Willebrand Factor is produced in rabbit using purified von Willebrand factor (factor VIII R:Ag) as the immunogen. |
| General description: | Human von Willebrand factor (factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes. |
| Immunogen: | von Willebrand Factor (Factor VIII-related antigen) from human plasma. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| Preparation Note: | Whole antiserum is purified to provide an IgG fraction of antiserum. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |