Anti-Human IgG (whole molecule)−FITC antibody produced in goat
SIGMA/F3512 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Goat Anti-Human IgG (whole molecule)
MDL Number: MFCD00162487
Product Type: Chemical
Immunofluorescence Hep-2 Human cells were probed and developed using 1:200 ANA (anti-nuclear antibody assays) test system followed by 1:32 Anti-Human IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F3512).
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:32-1:64 using Hep2 cells |
| Application: | Anti-Human IgG (whole molecule)-FITC antibody has been used: • in nucleolar staining • in single color fluorescent labeling for the detection of lytically induced cells • to detect the model antigen • in immunohistochemistry • in immunofluorescence |
| Application: | Anti-Human IgG (whole molecule)-FITC antibody may be used for anti-nuclear antibody (ANA) assays. The antibody may also be used in FACS-based assays. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Specificity of anti-human IgG (whole molecule)-FITC antibody for human IgG is determined by immunoelectrophoresis against purified human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins. |
| General description: | Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. The two heavy chains and two light chains of IgG are connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. |
| Immunogen: | Pooled normal human serum |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |