Monoclonal Anti-Cytokeratin, pan−FITC antibody produced in mouse
SIGMA/F3418 - clone C-11, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Pan Cytokeratin
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Yap- and Cdc42-dependent nephrogenesis and morphogenesis during mouse kidney development. By: Reginensi, A., Scott, R. P., et al. in PLoS Genet, 2013. PubMed ID: 23555292 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003380), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Yap- and Cdc42-dependent nephrogenesis and morphogenesis during mouse kidney development. By: Reginensi, A., Scott, R. P., et al. in PLoS Genet, 2013. PubMed ID: 23555292 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pgen.1003380), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Formalin-fixed, paraffin-embedded section of human placenta stained with 1:50 Monoclonal Anti-CytokeRatin, pan-FITC antibody produced in Mouse (Cat. No. F3418).
Immunofluorescence HeLa human cervix epitheloid carcinoma cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:50 Monoclonal Anti-CytokeRatin, pan-FITC antibody produced in Mouse (Cat. No. F3418). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence MCF7 human breast carcinoma cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:50 Monoclonal Anti-CytokeRatin, pan-FITC antibody produced in Mouse (Cat. No. F3418). Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence NRK normal Rat kidney kidney cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:50 Monoclonal Anti-CytokeRatin, pan-FITC antibody produced in Mouse (Cat. No. F3418). Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | C-11, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, mouse, frog, human, kangaroo rat, rat |
| storage condition | protect from light |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:50 using PtK2 cells |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human placenta | |
| western blot: suitable |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (1 paper) |
| Application: | Monoclonal Anti-Cytokeratin, pan−FITC antibody has been used in immunocytochemistry and has also been used to visualize lung epithelial cells. |
| Biochem/physiol Actions: | Cytokeratins helps in subtyping several kinds of epithelial differentiation or for narrowing possible sites of origin for metastatic carcinomas. Cytokeratins may play an important role in transhepatic transport and canalicular secretion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Cytokeratins are the intermediate filaments of endocrine cells. These proteins belong to the intermediate filament (10 nm) superfamily of cytoskeletal proteins. Cytokeratins are the largest and most complex group of intermediate filaments. The family consists of at least 30 proteins with molecular weights ranging from 40kDa to 68kDa. |
| Immunogen: | keratin-enriched preparation from cultured human epidermoid carcinoma cell line A431. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |