Anti-Fibroblast Growth Factor-Basic (1-24) antibody produced in rabbit
SIGMA/F3393 - fractionated antiserum, lyophilized powder
Synonym: Anti-FGF2; Anti-bFGF
MDL Number: MFCD01322020
Product Type: Chemical
Immunohistochemistry
Immunoperoxidase staining (PAP) of a fibrillary differentiated astrocytoma (WHO grade I, SAMS grade 1) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). High bFGF expression is shown within tumor cells as well as within endothelial cells of preexistent capillary blood vessels. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without an enzymatic or antigen demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover Germany.
Immunoperoxidase staining (PAP) of a fibrillary differentiated astrocytoma (WHO grade I, SAMS grade 1) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). High bFGF expression is shown within tumor cells as well as within endothelial cells of preexistent capillary blood vessels. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without an enzymatic or antigen demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover Germany.
Immunohistochemistry
Immunoperoxidase staining (PAP) of a gemistocytic differentiated area of an anaplastic astrocytoma (WHO grade III, SAMS grade 3) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). High bFGF expression is shown within tumor cells as well as within neoplastic transformed endothelial cells of capillary blood vessels. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without any enzymatic or antigen-demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany.
Immunoperoxidase staining (PAP) of a gemistocytic differentiated area of an anaplastic astrocytoma (WHO grade III, SAMS grade 3) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). High bFGF expression is shown within tumor cells as well as within neoplastic transformed endothelial cells of capillary blood vessels. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without any enzymatic or antigen-demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany.
Immunohistochemisrty
Immunoperoxidase staining (PAP) of U87MG glioma cell suspension implanted into nude rat brain (Han rnu/rnu) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). High bFGF expression is exclusively shown within tumor endothelial cells. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without any enzymatic or antigen-demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany. Ref: Stan, A.C., et al., J. Neurosurg., 82, 1044 (1995).
Immunoperoxidase staining (PAP) of U87MG glioma cell suspension implanted into nude rat brain (Han rnu/rnu) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). High bFGF expression is exclusively shown within tumor endothelial cells. The tissue sample was paraffin-embedded and 4% Tris-buffered formalin-fixed without any enzymatic or antigen-demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany. Ref: Stan, A.C., et al., J. Neurosurg., 82, 1044 (1995).
Immunohistochemistry
Immunoperoxidase staining (PAP) of a freshly explanted gliobastoma multiforme (WHO grade IV, SAMS grade 4) in culture (passage # 3) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). Intracytoplasmatically, homogeneous, high bFGF expression is shown within highly polymorphic tumor cells. The tumor cells on glass coverslips were methanol/acetone (100%, ice-cold, 1:1) fixed without any enzymatic or antigen-demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany.
Immunoperoxidase staining (PAP) of a freshly explanted gliobastoma multiforme (WHO grade IV, SAMS grade 4) in culture (passage # 3) using Rabbit Anti-Fibroblast Growth Factor-Basic (Cat. No. F3393). Intracytoplasmatically, homogeneous, high bFGF expression is shown within highly polymorphic tumor cells. The tumor cells on glass coverslips were methanol/acetone (100%, ice-cold, 1:1) fixed without any enzymatic or antigen-demasking pre-processing.
From Dr. A.-C. Stan, Institute of Neuropathology, Medical School Hannover, Hannover, Germany.
| antibody form | fractionated antiserum |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| Quality Level | 200  |
| species reactivity | human, bovine, rat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1:1,000 | |
| UniProt accession no. | P09038  |
| Analysis Note: | Bioactivity: Anti-Fibroblast Growth Factor-Basic (1-24) caused a dose-dependent decrease in the mitogenic activity of FGF-Basic such that a final dilution of 1:50 neutralized the bioactivity of 1 unit of FGF Basic. |
| Application: | Anti-Fibroblast Growth Factor-Basic (1-24) antibody produced in rabbit has been used in: • immunohistochemistry • immunostaining • immunoblotting |
| Biochem/physiol Actions: | Fibroblast Growth Factor Basic (also known as bFGF) is a pleotropic cytokine that that is a potent mitogenic factor. On binding to its cognate receptor tyrosine kinase, FGFR, bFGF is capable of activation of pathways such as MAPK and PI3K and produce cellular effects in varied cell types. Fibroblast Growth Factor-basic is reported to induce proliferation of fibroblasts, differentiation, wound healing and regeneration, limb formation, tissue remodeling and angiogenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Fibroblast growth factor-basic (FGFb), also known as NUDT6 (nudix (nucleoside diphosphate linked moiety X)-type motif 6) is encoded by the gene mapped to human chromosome 4q28.1. The FGFb gene spanning 34271 bp on genomic DNA contains 16 exons and six introns. FGFb RNA transcripts are expressed in various human tissues including liver, thymus, spleen, peripheral blood leukocytes, heart skeletal muscle, testis, colon and kidney. |
| Immunogen: | synthetic peptide sequence corresponding to bovine FGF2 (1-24). |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |