Fpg Protein from Escherichia coli
SIGMA/F3174 - ≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis
Synonym: DNA-(apurinic or apyrimidinic site)lyase MutM (APlyase MutM); Fapy-DNAglycosylase; Formamidopyrimidine-
CAS Number: 78783-53-6
MDL Number: MFCD02264758
Product Type: Chemical
SDS-PAGE Fpg Protein from Escherichia coli(Cat. No. F3174) was assessed on SDS-PAGE.
| application(s) | genomic analysis |
| assay | ≥90% (SDS-PAGE) |
| biological source | Escherichia coli |
| composition | protein, 0.1- 0.3 mg/mL Bradford |
| form | buffered aqueous glycerol solution |
| mol wt | 30.2 kDa (269 amino acids, predicted from the nucleotide sequence) |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | >20,000 units/mg protein |
| storage condition | (Tightly closed) |
| storage temp. | −20°C |
| technique(s) | nucleic acid detection: suitable |
| UniProt accession no. | P05523  |
| Application: | Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay. |
| Biochem/physiol Actions: | Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase. |
| Biochem/physiol Actions: | Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action. |
| Biochem/physiol Actions: | Fpg specifically acts on 3′- and 5′-phosphodiester bonds. |
| General description: | Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type. |
| General description: | Fpg contains two domains separated by a flexible hinge. Research area: Cell signaling |
| Other Notes: | One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C. |
| Packaging: | 10 μg in poly bottle |
| Physical form: | Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: >20,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.2.23 ( BRENDA | IUBMB ) |
| UNSPSC | 12352202 |