Monoclonal Anti-Actin–FITC antibody produced in mouse
SIGMA/F3046 - clone AC-40, purified immunoglobulin, buffered aqueous solution
Synonym: Monoclonal Anti-Actin
Product Type: Chemical
Immunofluorescence HS-68 Human foreskin fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained and developed with 1:75 Monoclonal Anti-Actin-FITC antibody produced in Mouse, Clone: AC-40 (Cat. No. F3046).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AC-40, monoclonal |
| conjugate | FITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts |
| Application: | Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts. |
| Application: | Monoclonal anti-actin antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. |
| Application: | Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. |
| Biochem/physiol Actions: | Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues. |
| General description: | The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel′s or Bouin′s solutions may also be used as fixatives. |
| Immunogen: | synthetic actin C-terminal peptide, attached to a Multiple Antigen Peptide (MAP) backbone |
| Other Notes: | To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/acti. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |