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Monoclonal ANTI-FLAG® M1 antibody

SIGMA/F3040 - clone M1, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-ddddk; Anti-dykddddk

MDL Number: MFCD00212941
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-F3040-.2MG 0.2 mg
 $625.00
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45-F3040-1MG 1 mg
 $1070.00
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45-F3040-5MG 5 mg
 $1970.00
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antibody form purified immunoglobulin
antibody product type primary antibodies
biological source (Bioreactor)
clone M1, monoclonal
concentration 2-5 mg/mL
conjugate unconjugated
form buffered aqueous solution
immunogen sequence DYKDDDDK
purified by using Protein A
Quality Level 200 
shipped in dry ice
species reactivity all
storage temp. −20°C
technique(s) western blot: 10 μg/mL
Application: Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.


Chromatin immunoprecipitation (1 paper) 


Immunofluorescence (1 paper) 

Learn more product details in our FLAG® applications  portal.
Biochem/physiol Actions: Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
General description: The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A
Immunogen: FLAG; peptide sequence DYKDDDDK
Legal Information: ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Legal Information: FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research 
Physical form: Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).
Preparation Note: Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.
Hazard Codes Xn
Risk Statements 21/22
Safety Statements 36/37
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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